微型膜片鉗-時(shí)間分辨的低電流檢測(cè)儀
作者: Kevin Peng 發(fā)布于: 2016-08-23 17:11 點(diǎn)擊:次
從單個(gè)細(xì)胞來(lái)看, 生命物質(zhì)的特征之一是細(xì)胞被一層膜所包圍。我們已經(jīng)知道, 活細(xì)胞的結(jié)構(gòu)比較復(fù)雜, 其中膜結(jié)構(gòu)實(shí)際上涉及到細(xì)胞活性的各個(gè)水平。例如, 在真核細(xì)胞中, 膜充滿(mǎn)了細(xì)胞, 出現(xiàn)在線(xiàn)粒體, 高爾基體和細(xì)胞核等。這些細(xì)胞膜起到把細(xì)胞質(zhì)分開(kāi)并為生命過(guò)程的發(fā)展提洪骨架的作用。盡管細(xì)胞膜有內(nèi)外之分, 但一般認(rèn)為它們是互相連通的。人們常把蛋白質(zhì)或核酸等看作基本“ 生命” 實(shí)體, 但從更多的證據(jù)來(lái)這些細(xì)胞要素在某些類(lèi)型的膜把它們從周?chē)h(huán)境中分離開(kāi)以前是不會(huì)發(fā)生更多轉(zhuǎn)化作用的。由于許多生理和生物化學(xué)作用不是發(fā)生在大量細(xì)胞質(zhì)內(nèi), 而是發(fā)生在各種細(xì)胞膜中, 因此生物膜便成為生物化學(xué)和生物物理學(xué)所研究的核心問(wèn)題之一，這個(gè)時(shí)候我們就需要膜片鉗。
:膜片鉗技術(shù)是在電壓鉗技術(shù)的基礎(chǔ)上發(fā)展起來(lái)的, 來(lái)反映細(xì)胞膜上離子通道活動(dòng)的一項(xiàng)技術(shù)。它對(duì)通過(guò)離子通道的離子電流進(jìn)行記錄, 目前已發(fā)展出多種記錄模式, 廣泛應(yīng)用于神經(jīng)科學(xué)、藥理學(xué)、細(xì)胞生物學(xué)、生理學(xué)和分子生物學(xué)等學(xué)科領(lǐng)域。
膜片鉗技術(shù)(patch-clamptechnique)是在電壓鉗技術(shù)的基礎(chǔ)上發(fā)展起來(lái)的, 采用記錄流過(guò)離子通道的離子電流, 來(lái)反映細(xì)胞膜上單一的(或多個(gè)的)離子通道分子活動(dòng)的技術(shù)。該技術(shù)可將一尖端經(jīng)加熱拋光的玻璃微電極管吸附只有幾平方微米的細(xì)胞膜, 在玻璃電極尖端邊緣與細(xì)胞膜之間形成高阻封接, 因而可通過(guò)微電極直接對(duì)膜片(細(xì)胞膜小區(qū)域)進(jìn)行電壓鉗制,而無(wú)需使用其它微電極(如圖)。1976年, 德國(guó)生理學(xué)家Neher和Sakmann[ 2] 首先利用此技術(shù)研究肌肉細(xì)胞膜上的乙酰膽堿受體通道, 記錄出了量值在皮安級(jí)(10-12 A)的微弱電流。
基于元素的設(shè)計(jì)定制芯片(ASIC)使用標(biāo)準(zhǔn)和低成本的CMOS工藝。多年的經(jīng)驗(yàn)在微電子設(shè)計(jì)應(yīng)用于若設(shè)備給團(tuán)隊(duì)一個(gè)獨(dú)特的元素,在非常低振幅的信號(hào)讀出獨(dú)占優(yōu)勢(shì)。元素微芯片可以測(cè)量微微安培電流(10 - 12)與帶寬高達(dá)數(shù)百千赫范圍內(nèi)由于深知識(shí)達(dá)到單元素和多通道信號(hào)采集非常低振幅信號(hào),低功率管理技術(shù)、模擬數(shù)字轉(zhuǎn)換和數(shù)字?jǐn)?shù)據(jù)精化。
公司開(kāi)發(fā)了單通道和四通道兩種微電流放大器，廣泛應(yīng)用于電化學(xué)，生物物理，細(xì)胞檢測(cè)，生物光子學(xué)，生理學(xué)，納米電極，納米通道 等領(lǐng)域，被業(yè)界認(rèn)可，被很多膜片鉗的檢測(cè)系統(tǒng)廠(chǎng)家采用，具有價(jià)格便宜，體積小，便于構(gòu)建等優(yōu)點(diǎn)，可適用于廣大科研用戶(hù)和OEM客戶(hù)。
eONE – Miniaturized One-Channel Amplifier
The world’s smallest low-cost current amplifier for nanopores and single-channel electrophysiology applications. eONE is a complete, USB-powered, one-channel data acquisition system. It incorporates the low noise amplifier, the digitizer and filters, and the voltage stimulus generator, all in a very small footprint (only 30x15x74mm). It is suitable for research and educational activities which use bilayer lipid membranes (BLM) or whole cells.
A small, easy-to-use, USB plug ‘n play system for acquiring very low-current signals!
Simple experimental setup
How to connect eONE in a typical electrophysiology setup
Orbit mini - World's only 'hand-held' four-channel bilayer setup
Thanks to the collaboration with Nanion Technologies and Ionera, the Orbit mini, the world's only handheld four-channel bilayer setup, has been developed. The core of the system is eFOUR, the smallest four-channel, USB-powered, current amplifier designed and produced by Elements.
A compact and complete BLM educational kit for accurate electrophysiological recordings. The kit includes the eONE amplifier, the EDR software with real-time analysis and all the tools for fast screening of ion-channels functional activity in lipid environments.
The kit is contained into a 16 x 10 x 5 cm Faraday cage and includes:
• a recording chamber made by two 1 ml Delrin cuvette with electrode input;
• a set of laser drilled Teflon membranes with fixed hole size of 50, 80, 110 µm;
• paint brush, Ag wires, electrode connectors to plug the eONE amplifier.
A large number of standard experiments can be performed using the BLMkit. Stable membranes can be easily formed to study ion channels or toxin property. Without bulky setup you can start to make BLM experiments on your desk. The BLMkit is especially design for teaching activity for lab experience, to enable students to learn electrophysiology basis on Lipid Bilayers property, membrane protein study and ion channels analysis.
EDA - Elements Data Analyzer
The Elements Data Analyzer software enables users to open and visualize the current and voltage traces acquired by Elements Data Reader in the .dat and .abf formats. The intuitive graphical user interface allows a quick and easy data display.
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